ABSTRACT
Background: Endometriosis is a complex disorder in reproductive age women which consist of stromal and epithelial cells implantation outside the uterine cavity. Adiponectin is a member of cytokine family with various metabolic roles and proliferation inhibition of many cancer cells. The aim of the present research was to determine adiponectin effect on human endometriotic stromal cells [ESCs] proliferation and their expression of adiponectin receptors
Methods: In this experimental study, endometrial biopsies [n=7] were taken. ESCs isolation was done by enzymatic digestion and cell filtrations. ESCs of each biopsy were divided into four groups: 0 [control], 10, 100, and 200 ng/ml adiponectin concentrations in three different times [24, 48, or 72 h]. The effect of adiponectin on ESC viability and expression of mRNA Adipo receptorl [Rl] and Adipo receptor2 [R2] was determined by Trypan blue staining and semi-quantitative RT-PCR, respectively. Data were analyzed by one-way ANOVA and unpaired student's t-test, and P<0.05 was considered statistically significant
Results: Adiponectin inhibited human endometriotic stromal cell proliferation in time- and dose-dependent manners significantly [P=0.001]. Expression of AdipoRl and AdipoR2 gene receptors was increased in human ESCs significantly [P<0.05]. Adiponectin can suppress endometriosis by inhibiting ESC proliferation and increased AdipoRl and AdipoR2 expression
ABSTRACT
Adiponectin is one of the most important adipokines secreted from fatty tissue that has a direct inhibitory effect on the development of cancer cells. Adiponectin plays an important role in human reproduction system and fertility of women. Adiponectin concentration decreases in women with endometriosis and endometrial cancer. The aim of the present study was to investigate the effect of adiponectin on human endometrial stromal cell [HESC] viability as well as mRNA expression of Adipo R1 and Adipo R2 receptors. In this experimental study, eight endometrial biopsies were taken and stromal cells were separated by enzymatic digestion and cell filtrations. Stromal cells of each biopsy were divided into four groups: control, 10, 100, and 200 ng/ml adiponectin concentrations. The effect of adiponectin on viability of the normal HESCs was studied by trypan blue staining and the relative expression levels of Adipo R1 and R2 were analyzed by semi-quantitative reverse transcription polymerase chain reaction [RT-PCR]. Data were analyzed by one way ANOVA and unpaired student's t test and p<0.05 was considered significant. Adiponectin decreased viability of normal human endometrial stromal cells in a dose and time dependent manner. Expression of Adipo R1 and Adipo R2 receptors did not change in the presence of adiponectin. Adiponectin can directly influence the viability of HESCs and decrease their viability, but it didn't change expression of adiponectin receptors